共聚焦型离体微血管张力测定系统 – 360CW 用于与光学成像系统结合使用,让光学镜头近距离靠近微血管样本,从而允许激光共聚焦显微镜(LSCM)进行荧光染料或标记物进行高分辨率成像。该设备巧妙地将LSCM与微血管张力器浴槽相结合,同时测量等长收缩和荧光成像。一个典型案例是测量离体微血管等长收缩时力学变化与血管平滑肌细胞内Ca2+测量相关联。
独特的开放式锥型浴槽设计,可在XYZ三个方向调节样本位置,确保更精确地调整样本与物镜的位置。 浴槽设计允许倒置显微镜上使用的高数值孔径浸没物镜和标准直立显微镜上使用的镜头直接浸没。此外,特殊的样本支架被设计为允许样本血管贴近窗口上方,物镜工作距离达到250 μm。浴槽配有倒置或正置显微镜定制的浴槽盖。浴槽盖上含有废液抽吸、通气或测量电极(pH、NO、O2)接口。 化合物可直接添加到浴槽中,并可在专用显微镜上直接测量血管张力。可选择安装DMT设备软件驱动,USB线直接连接电脑输出数字信号,通过LabChart软件采集数字信号。 |
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